people: R. Datar

Dr. Ram Datar

Dr. Ram Datar is a cancer molecular pathologist and a Senior Scientist in the Biosciences Division at ORNL.

He is also the author of 4 joint patents (pending), several invention disclosure agreements. Apart from his lab work, Dr Datar works as an editor of the journal Current Issues in Molecular Biology, and is also a reviewer for a number of biomedical journals.

Prior to his arrival at ORNL, he was Faculty in the Department of Pathology at the University of Southern California from 1998-2007 and for the Department of Atomic Energy in the Government of India for several years.

Dr Datar received his Ph. D. in Applied Biology from the University of Bombay in 1996 with a thesis entitled Gene Regulation in Oral Cancers

Prior to his Ph.D work Dr Datar completed two masters’ degrees in Molecular Biology (MS and MPhil), and a B.S. in Zoology in 1984, 1981, and 1979 respectively from Pune University (India).

Current and Past Projects

• Development of Microfabricated Devices for Capture and Characterization of Circulating Tumor Cells in Cancer Patient Blood Samples:
• Development of Opto-mechanical Chips for Molecular Profiling of Cancer:
• Development of Nanocapillary Electrophoretic Analysis Technology:
• Analysis of Bone Marrow & Sentinel Node Occult metastases in Breast Cancer
• Detection of Occult Metastases in Patients with Lung Cancer:
• Analysis of Cellular and Molecular Pathways of Bladder Cancer progression:
• Development of antigen retrieval-based technologies for Retrieval of DNA, RNA and Protein from Archival Tissues:
• Detection and Analysis of Mechanisms of Androgen Ablation Insensitivity in Prostate Cancer:

Publications

KS Nadkarni, RH Datar and SGA Rao. “Antisense RNA Therapy for CML- A Hypothesis” Med Hypotheses, 35: 305-09, 1991

SA Imam, MS Kim, L Anker, RH Datar, RE Law and CR Taylor. “Systematic determination of telomerase activity and telomeric length during the progression of human breast cancer in cell culture model”, Anticancer Res, 17(6D), 4435-41, 1997

M Ozen, SA Imam, RH Datar, AS Multani, R Narayanan, LW Chung, AC von Eschenbach and S Pathak. “Telomeric DNA: Marker for human prostate cancer development?” The Prostate, 36(4), 264-71, 1998

RH Datar, A Reles, PL Li, D Tsao-Wei, SL Groshen, CR Taylor and SA Imam. “Analysis of telomerase activity in ovarian cystadenomas, low malignant potential ovarian tumors and ovarian carcinomas.” Gynecol Oncol, 74, 338-345, 1999

WN Naritoku, RH Datar, PL Li, SL Groshen, CR Taylor and SA Imam. “Telomerase activity: Its comparison for the detection of tumor cells in fine-needle aspiration and biopsy specimens.” Cancer Cytopathol, 87 (4), 210-215, 1999

RH Datar. “Nucleic Acids in Disease (I): Information Launch-pad” Part I of an invited Review Series “Nucleic Acids in Medicine”, Nat Med J India, 13(5), 255-62, 2000.

G Wu, K Hansen, T Thundat, RJ Cote, RH Datar, A. Chakraborty, A Majumdar. “Control and manipulation of nanomechanical motion generated from biomolecular interactions.” Proceedings of National Academy of Sciences, USA, 98(4), 1560-1564, 2001.

K Hansen, HF Ji, G. Wu, RH Datar, T Thundat, RJ Cote, A Majumdar. “Effect of single nucleotide polymorphisms (SNPs) on Nanomechanical Motion.” Anal Chem, 73, 1567-1571, 2001.

RH Datar, NN Joshi. “Nucleic Acids in Medicine (II): Nucleic Acids In Diagnosis"”, Nat Med J India, 14(1):34-39, 2001.

RH Datar. “Nucleic Acids in Medicine (III) - Nucleic Acids In Diagnosis: (Part II): Genetic Testing and Screening” Nat Med J India, 14(2): 93-98, 2001

B George, RJ Cote, RH Datar. “Nucleic Acids in Medicine (IV): Nucleic Acids in Prognosis.” Nat Med J India, 14(3): 163-168, 2001

G Wu, RH Datar, KM Hansen, T Thundat, RJ Cote, A Majumdar. Nanomechanical biosensor for sensitive and specific detection of prostate specific antigen (PSA). Nature Biotechnology, 19(9): 956-960, 2001

RH Datar. “Nucleic Acids in Medicine (V): Nucleic Acids In Therapy”, Nat Med J India, 14(5): 290-296, 2001

RH Datar. “Nucleic Acids: In the Future, In Commerce and in the Laboratory” Nat Med J India, 14(6): 349-354, 2001

V Pagliarulo, RH Datar, RJ Cote. “Genetic and Expression Profiling in Pharamcogenomics: The Changing Face of Medicine.” Curr. Issues Mol. Biol. 4: 101-110, 2002

SR Shi, RJ Cote, L Wu, C Liu, R Datar, Y Shi, D Liu, H Lim, CR Taylor. “DNA extraction from archival formalin-fixed, paraffin-embedded tissue sections based on the antigen retrieval principle: heating under the influence of pH.” Journal of Histochemistry & Cytochemistry. 50(8):1005-11, 2002

RJ Cote and RH Datar. “Therapeutic Approaches to Bladder Cancer: Identifying targets and mechanisms.” Crit Rev Oncol / Hematol, 46, S67-S83, 2003

SJ Chatterjee, SR Shi, RH Datar, PJ Goebell, YK Fung, PA Jones, C Cordon-Cardo, RJ Cote. “Hyperphosphorylation of pRb: a mechanism for RB tumor suppressor pathway inactivation in bladder cancer.” J Pathol, 203: 762–770, 2004

SJ Chatterjee, RH Datar, D Youssefzadeh, B George, JP Stein, L Young, SR Shi, C Gee, SG Groshen, DG Skinner, RJ Cote. “The combined effects of Rb, p21, and p53 expression in the progression of bladder transitional cell carcinoma.” J Clin Oncol 22, (6) 1007-1013, 2004

D Liu, RJ Cote, S DeVries, F Waldman, RH Datar. “LM-PCR Permits Highly Representative Whole Genome Amplification in Small Number of Microdissected Cells and Paraffin-Embedded Tumor Tissue Sections.” Diagn Mol Pathol 13:105–115, 2004

V Pagliarulo, B George, S Groshen, P Laird, J Cai, J Willey, R Datar, RJ Cote. “Sensitivity and reproducibility of standardized competitive RT PCR for transcript quantification and its comparison with real time PCR.” Mol Cancer 3:5-15, 2004

SR Shi, RJ Cote, RH Datar, C Liu, L Wu, Z Zhang, CR Taylor. “DNA extraction from archival formalin-fixed, paraffin-embedded tissue section: Heat-induced retrieval in alkaline solution.” Histochem Cell Biology 122-211-218, 2004

B George, RJ Cote, RH Datar. “Commentary on: Does the Decatenation G2 Checkpoint Suppress Bladder Carcinogenesis?” Am J Urol Reviews, 2 (7), 347-350, 2004

RJ Cote, PW Laird, RH Datar. “Another Therapeutic Target Emerges in Urothelial Cancer: Promoter Hypermethylatin”. Editorial Commentary in J Clin Oncol, 23(13):2879-81, 2005

AP Mitra, RH Datar and RJ Cote. “Molecular Staging of Bladder Cancer”. Br J Urol, 96(1):7-12, 2006.

Y Shi, SJ Chatterjee, FH Brands, SR Shi, L Pootrakul, CR Taylor, RH Datar, RJ Cote. The role of Coordinate Molecular Alterations in the Development of Androgen Independent Prostate Cancer: An in vitro Model that Corroborates Clinical Observations. Br J Urol Int, In Press, 2006

C Li, M Curreli, H Lin, B Lei, FN Ishikawa, RH Datar, RJ Cote, ME Thompson, C Zhou. Complementary detection of prostate-specific antigen using In2O3 nanowires and carbon nanotubes. J Am Chem Soc;127(36):12484-5, 2005

M Balic, N Dandachi, H Lin, RH Datar. Cancer metastasis: Advances in the detection and characterization of disseminated tumour cells facilitate clinical translation. Natl Med J India;18:250-5, 2005

A Mitra, H Lin, RJ Cote, RH Datar. “Biomarker profiling for cancer diagnosis, prognosis and therapeutic management” Natl Med J India, 18(6):304-12, 2006.

A Mitra, RH Datar, RJ Cote. “Molecular pathways in invasive bladder cancer: new insights into mechanisms, progression, and target identification”. Invited Review for Special Urologic Oncology Review Issue, J Clin Oncol, 10;24(35):5552-64, 2006

A Mitra, H Lin, RH Datar, RJ Cote. “Molecular biology of bladder cancer: Translation into clinical medicine” Clin Genitourinary Cancer, 5(1):67-77, 2006

MA Thorat, RH Datar, Joshi NN. “Cancer immunotherapeutics: raising the ante”. Natl Med J India.19(3):140-50, 2006

H Lin, RH Datar. “Medical applications of nanotechnology”. Natl Med J India. 19(1):27-32, 2006

ML Gonzalgo, RH Datar, MP Schoenberg, RJ Cote. “DNA Methylation in Development of Bladder Cancer and Its Role in Diagnosis and Prognosis of Bladder Cancer” Urol Oncol Sem Original Invest, 25(3):228-35, 2007

M Balic, HL Lin, RH Datar, L Young, Hawes, A Guiliano, G McNamara., RJ Cote. “The majority of early disseminated cancer stem cells detected in bone marrow of breast cancer patients have a putative stem/ progenitor cell phenotype” Clin Cancer Res. 2006 Oct 1;12(19):5615-21

L Pootrakul, RH Datar, SR Shi, S Groshen, J Cai, A Lee, RJ Cote “GRP78 Positivity is Associated with Androgen Independent Status in Advanced Prostate Cancer” Clin Cancer Research, 12(20 Pt 1):5987-93, 2006

B George, SJ Beil, RH Datar, L Wu, N Patten, J Cai, S Groshen, RJ Cote “Functional Role of p53 Gene Mutations and Protein Nuclear Positivity in the Response of Invasive Bladder Cancer to Chemotherapy.” J Clin Oncol, In Press, 2007

L Pootrakul, RH Datar, SR Shi, S Groshen, J Cai, A Lee, RJ Cote “GRP78 Positivity is Associated with Androgen Independent Status in Advanced Prostate Cancer” Clin Cancer Res 12(20 Pt 1):5987-93, 2006

A Mitra, A Almaal, B George, V Pagliarulo, D Fry, P Lenehan, W Worzel, RJ Cote, RH Datar. “Use of machine learning algorithms in predicting lymph node status of bladder cancer tumors analyzed by standardized competitive RT PCR.” BMC Cancer;6:159, 2006

S Zheng, H Lin, J-Q Liu, M Balic, R Datar, RJ Cote, Y-C Tai. “Membrane microfilter device for selective capture, electrolysis and genomic analysis of human circulating tumor cells”. J Chromatogr A. 2007 May 29; [Epub ahead of print]

M Casimiro, O Rodriguez, L Pootrakul, M Aventian, N Lushina, C Cromelin, G Ferzli, K Johnson, S Fricke, F Diba, B Kallakury, C Ohanyerenwa, M Chen, M Ostrowski, MC Hung, SA Rabbani, R Datar, R Cote, R Pestell, C Albanese. “ErbB-2 induces the cyclin D1 gene in prostate epithelial cells in vitro and in vivo” Cancer Res. 2007;67(9):4364-72.

Book

Concepts in Applied Molecular Biology, Published by Eaton Publications, Informa Biosciences Group, USA., ISBN 1-881299-30-9, 2003